Expression of glutathione S-transferases in poplar trees (Populus trichocarpa) exposed to 2,4,6-trinitrotoluene (TNT).
Identifieur interne : 003923 ( Main/Exploration ); précédent : 003922; suivant : 003924Expression of glutathione S-transferases in poplar trees (Populus trichocarpa) exposed to 2,4,6-trinitrotoluene (TNT).
Auteurs : Laura B. Brentner [États-Unis] ; Sachiyo T. Mukherji ; Kate M. Merchie ; Jong Moon Yoon ; Jerald L. Schnoor ; Benoit Van AkenSource :
- Chemosphere [ 0045-6535 ] ; 2008.
Descripteurs français
- KwdFr :
- 2,4,6-Trinitro-toluène (métabolisme), 2,4,6-Trinitro-toluène (pharmacologie), ADN des plantes (métabolisme), Dépollution biologique de l'environnement (MeSH), Explosifs (métabolisme), Explosifs (pharmacologie), Glutathione transferase (génétique), Glutathione transferase (métabolisme), Gènes de plante (MeSH), Génome végétal (MeSH), Populus (effets des médicaments et des substances chimiques), Populus (enzymologie), Populus (métabolisme), Régulation de l'expression des gènes végétaux (MeSH).
- MESH :
- effets des médicaments et des substances chimiques : Populus.
- enzymologie : Populus.
- génétique : Glutathione transferase.
- métabolisme : 2,4,6-Trinitro-toluène, ADN des plantes, Explosifs, Glutathione transferase, Populus.
- pharmacologie : 2,4,6-Trinitro-toluène, Explosifs.
- Dépollution biologique de l'environnement, Gènes de plante, Génome végétal, Régulation de l'expression des gènes végétaux.
English descriptors
- KwdEn :
- Biodegradation, Environmental (MeSH), DNA, Plant (metabolism), Explosive Agents (metabolism), Explosive Agents (pharmacology), Gene Expression Regulation, Plant (MeSH), Genes, Plant (MeSH), Genome, Plant (MeSH), Glutathione Transferase (genetics), Glutathione Transferase (metabolism), Populus (drug effects), Populus (enzymology), Populus (metabolism), Trinitrotoluene (metabolism), Trinitrotoluene (pharmacology).
- MESH :
- chemical , genetics : Glutathione Transferase.
- chemical , metabolism : DNA, Plant, Explosive Agents, Glutathione Transferase, Trinitrotoluene.
- chemical , pharmacology : Explosive Agents, Trinitrotoluene.
- drug effects : Populus.
- enzymology : Populus.
- metabolism : Populus.
- Biodegradation, Environmental, Gene Expression Regulation, Plant, Genes, Plant, Genome, Plant.
Abstract
Twelve Populus genes were identified from Arabidopsis thaliana sequences previously shown to be induced by exposure to 2,4,6-trinitrotoluene (TNT). Using the resources of the Poplar Genome Project and National Center for Biotechnology Information databases, Populus conserved domains were identified and used to design gene specific primers. RNA extracted from root tissues of TNT-exposed hydroponic poplar plants was used to quantify the expression of genes by reverse-transcriptase real-time polymerase chain reaction. Cyclophilin and 18S ribosomal DNA genes were used as internal standards. Exposure to TNT resulted in a significant increase of gene expression of two glutathione S-transferases (GST), peaking at levels of 25.0 +/- 13.1 and 10 +/- 0.7 fold the expression level of non-exposed plants after 24 h for each of the GST genes, respectively. This paper demonstrates the use of functional genomics information from the model plant species, Arabidopsis, to identify genes which may be important in detoxification of TNT in the model phytoremediation species, Populus trichocarpa.
DOI: 10.1016/j.chemosphere.2008.07.059
PubMed: 18774158
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Schnoor</name></author><author><name sortKey="Van Aken, Benoit" sort="Van Aken, Benoit" uniqKey="Van Aken B" first="Benoit" last="Van Aken">Benoit Van Aken</name></author></analytic><series><title level="j">Chemosphere</title><idno type="ISSN">0045-6535</idno><imprint><date when="2008" type="published">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biodegradation, Environmental (MeSH)</term><term>DNA, Plant (metabolism)</term><term>Explosive Agents (metabolism)</term><term>Explosive Agents (pharmacology)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Genes, Plant (MeSH)</term><term>Genome, Plant (MeSH)</term><term>Glutathione Transferase (genetics)</term><term>Glutathione Transferase (metabolism)</term><term>Populus (drug effects)</term><term>Populus (enzymology)</term><term>Populus (metabolism)</term><term>Trinitrotoluene (metabolism)</term><term>Trinitrotoluene (pharmacology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>2,4,6-Trinitro-toluène (métabolisme)</term><term>2,4,6-Trinitro-toluène (pharmacologie)</term><term>ADN des plantes (métabolisme)</term><term>Dépollution biologique de l'environnement (MeSH)</term><term>Explosifs (métabolisme)</term><term>Explosifs (pharmacologie)</term><term>Glutathione transferase (génétique)</term><term>Glutathione transferase (métabolisme)</term><term>Gènes de plante (MeSH)</term><term>Génome végétal (MeSH)</term><term>Populus (effets des médicaments et des substances chimiques)</term><term>Populus (enzymologie)</term><term>Populus (métabolisme)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Glutathione Transferase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>DNA, Plant</term><term>Explosive Agents</term><term>Glutathione Transferase</term><term>Trinitrotoluene</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Explosive Agents</term><term>Trinitrotoluene</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Glutathione transferase</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>2,4,6-Trinitro-toluène</term><term>ADN des plantes</term><term>Explosifs</term><term>Glutathione transferase</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>2,4,6-Trinitro-toluène</term><term>Explosifs</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biodegradation, Environmental</term><term>Gene Expression Regulation, Plant</term><term>Genes, Plant</term><term>Genome, Plant</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Dépollution biologique de l'environnement</term><term>Gènes de plante</term><term>Génome végétal</term><term>Régulation de l'expression des gènes végétaux</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Twelve Populus genes were identified from Arabidopsis thaliana sequences previously shown to be induced by exposure to 2,4,6-trinitrotoluene (TNT). Using the resources of the Poplar Genome Project and National Center for Biotechnology Information databases, Populus conserved domains were identified and used to design gene specific primers. RNA extracted from root tissues of TNT-exposed hydroponic poplar plants was used to quantify the expression of genes by reverse-transcriptase real-time polymerase chain reaction. Cyclophilin and 18S ribosomal DNA genes were used as internal standards. Exposure to TNT resulted in a significant increase of gene expression of two glutathione S-transferases (GST), peaking at levels of 25.0 +/- 13.1 and 10 +/- 0.7 fold the expression level of non-exposed plants after 24 h for each of the GST genes, respectively. This paper demonstrates the use of functional genomics information from the model plant species, Arabidopsis, to identify genes which may be important in detoxification of TNT in the model phytoremediation species, Populus trichocarpa.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">18774158</PMID><DateCompleted><Year>2008</Year><Month>12</Month><Day>22</Day></DateCompleted><DateRevised><Year>2008</Year><Month>11</Month><Day>07</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0045-6535</ISSN><JournalIssue CitedMedium="Print"><Volume>73</Volume><Issue>5</Issue><PubDate><Year>2008</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Chemosphere</Title><ISOAbbreviation>Chemosphere</ISOAbbreviation></Journal><ArticleTitle>Expression of glutathione S-transferases in poplar trees (Populus trichocarpa) exposed to 2,4,6-trinitrotoluene (TNT).</ArticleTitle><Pagination><MedlinePgn>657-62</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.chemosphere.2008.07.059</ELocationID><Abstract><AbstractText>Twelve Populus genes were identified from Arabidopsis thaliana sequences previously shown to be induced by exposure to 2,4,6-trinitrotoluene (TNT). Using the resources of the Poplar Genome Project and National Center for Biotechnology Information databases, Populus conserved domains were identified and used to design gene specific primers. RNA extracted from root tissues of TNT-exposed hydroponic poplar plants was used to quantify the expression of genes by reverse-transcriptase real-time polymerase chain reaction. Cyclophilin and 18S ribosomal DNA genes were used as internal standards. Exposure to TNT resulted in a significant increase of gene expression of two glutathione S-transferases (GST), peaking at levels of 25.0 +/- 13.1 and 10 +/- 0.7 fold the expression level of non-exposed plants after 24 h for each of the GST genes, respectively. This paper demonstrates the use of functional genomics information from the model plant species, Arabidopsis, to identify genes which may be important in detoxification of TNT in the model phytoremediation species, Populus trichocarpa.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Brentner</LastName><ForeName>Laura B</ForeName><Initials>LB</Initials><AffiliationInfo><Affiliation>Department of Civil and Environmental Engineering, The University of Iowa, Iowa City, IA 52242.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mukherji</LastName><ForeName>Sachiyo T</ForeName><Initials>ST</Initials></Author><Author ValidYN="Y"><LastName>Merchie</LastName><ForeName>Kate M</ForeName><Initials>KM</Initials></Author><Author ValidYN="Y"><LastName>Yoon</LastName><ForeName>Jong Moon</ForeName><Initials>JM</Initials></Author><Author ValidYN="Y"><LastName>Schnoor</LastName><ForeName>Jerald L</ForeName><Initials>JL</Initials></Author><Author ValidYN="Y"><LastName>Van Aken</LastName><ForeName>Benoit</ForeName><Initials>B</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Chemosphere</MedlineTA><NlmUniqueID>0320657</NlmUniqueID><ISSNLinking>0045-6535</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018744">DNA, Plant</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D053834">Explosive Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>118-96-7</RegistryNumber><NameOfSubstance UI="D014303">Trinitrotoluene</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.5.1.18</RegistryNumber><NameOfSubstance UI="D005982">Glutathione Transferase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001673" MajorTopicYN="N">Biodegradation, Environmental</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018744" MajorTopicYN="N">DNA, Plant</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D053834" MajorTopicYN="N">Explosive Agents</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="Y">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017343" MajorTopicYN="N">Genes, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018745" MajorTopicYN="N">Genome, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005982" MajorTopicYN="N">Glutathione Transferase</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014303" MajorTopicYN="N">Trinitrotoluene</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2008</Year><Month>01</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2008</Year><Month>07</Month><Day>08</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2008</Year><Month>07</Month><Day>10</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2008</Year><Month>9</Month><Day>9</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2008</Year><Month>12</Month><Day>23</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2008</Year><Month>9</Month><Day>9</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">18774158</ArticleId><ArticleId IdType="pii">S0045-6535(08)00908-9</ArticleId><ArticleId IdType="doi">10.1016/j.chemosphere.2008.07.059</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Iowa</li></region></list><tree><noCountry><name sortKey="Merchie, Kate M" sort="Merchie, Kate M" uniqKey="Merchie K" first="Kate M" last="Merchie">Kate M. Merchie</name><name sortKey="Mukherji, Sachiyo T" sort="Mukherji, Sachiyo T" uniqKey="Mukherji S" first="Sachiyo T" last="Mukherji">Sachiyo T. Mukherji</name><name sortKey="Schnoor, Jerald L" sort="Schnoor, Jerald L" uniqKey="Schnoor J" first="Jerald L" last="Schnoor">Jerald L. Schnoor</name><name sortKey="Van Aken, Benoit" sort="Van Aken, Benoit" uniqKey="Van Aken B" first="Benoit" last="Van Aken">Benoit Van Aken</name><name sortKey="Yoon, Jong Moon" sort="Yoon, Jong Moon" uniqKey="Yoon J" first="Jong Moon" last="Yoon">Jong Moon Yoon</name></noCountry><country name="États-Unis"><region name="Iowa"><name sortKey="Brentner, Laura B" sort="Brentner, Laura B" uniqKey="Brentner L" first="Laura B" last="Brentner">Laura B. Brentner</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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